® reader to the computer. Cuvette port functionality can also be controlled using SoftMax Pro Software. Microplate reader settings. Molecular Devices Corporation. The drawing shows a standard microplate of 15 mm height covered with a lid. Most papers they cultured bacteria to OD600>0. 1. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Methods Preparation of reagents and standards. SpectraMax M5 microplate reader (Molecular Devices, California, USA) with excitation at 450 nm and emission at 570 nm. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Maximum signal with lowest background. The assay window spanned four orders of magnitude. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Fluorescence Microplate Reader Comparison Chart. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. The following document is intended to demonstrate setup of this instrument. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Pick the best speed, sensitivity, and price for your needs. Cables. M3-M5e_manual. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Figure 1. 0 μL) instead of the enzyme solution was used as the sample blank group. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. KEY FEATURES • Five. The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. SpectraMax® iD5. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). Wash Buffer Concentrate was diluted 10-fold. Published Dec 16, 2020 | Updated Dec 16, 2020. After 16 hr of treatment, cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Table of Contents. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. ,. 1. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. 16S rRNA gene sequencing . Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Molecular Devices SpectraMax™ Plus 384 Microplate Reader (s) with One-Year Warranty $9,000. The cells were then treated to induce apoptosis. The guide also covers the software features, the specifications, and the accessories of the systems. Spectramax M5 microplate reader: Molecular Devices: N/A:. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Protocol Name Version 6. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Compatible automation solutions for SpectraMax Readers. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. 1** Version 7. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Wide range of applications. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). 0 out of 5 $ 2,499 $ 1,999. 0 . 4. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. Parallel negative control assays had primary, secondary, or both antibodies omitted. used. These robust readers have been placed in labs from Antarctica to. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Shimadzu 2450 UV–Vis spectrophotometer and SpectraMax M5 microplate reader were used to perform the optical characterization and measurements. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. Fluorescence intensity was detected by. SpectraMax® Aquamax. Sale price $18,499. The absorbance value of each reaction solution was measured at 405 nm by a Molecular Device SpectraMax M5 microplate reader (Sunnyvale, CA, USA). Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. We use Spectramax M5 microplate reader in our lab. . flexibility of monochromator-based systems. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. keeping with best practices, reader performance should. Note: In this user guide, SpectraMax ® refers to several SpectraMax . book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Turn on the microplate reader. Shop now. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 40266 Email: drugdiscoverytech@lifetech. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. 3 mg. Detailed specifications of each model reader are reported in Section 7, “Specifications”. IMAP FP Settings in SoftMax Pro M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. When the user applies a set temperature of 37°C, the top is heated to 37. 5. Western blot analyses. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. B: Selection of Excitation Wavelength. #M5) SpectraMax® L Microplate Reader (cat. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. SpectraMax M5 Multi-Mode Microplate Reader. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Category 2 and 3 microplate readers use gradient heating as well. Do not touch the clear surfaces of the cuvette. 0 and 0. Figure 1. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. SpectraMax® ABS. 4. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. Supplier: Molecular Devices. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Choose cables that meet the requirement described in this appendix. FlexStation™ I. Nontreated cells in DMEM medium were used as a control. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. You should get comparable results from top and bottom reads using EC50's to compare reads. The drawing shows a standard microplate of 15 mm height covered with a lid. , Sunny-vale, CA). The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. M3-M5e_manual. Western blotting assay. edu) to be added as a trained user in iLab. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). 1 is GxP edition only; Protocols for the ABS1 Validation Plate. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Make sure the microplate is flat against. Five preferred detection modes. Optical absorbance. In brief, RAW 264. , Sunnyvale, CA) using the bottom well-scan mode where nine readings. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. 87. Molecular Devices Spectramax M5 Microplate Reader. Introduction. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. 49 2. 95. IMAP assays are based on binding of. Microplate Readers are modular and . This application note discusses the principles on which PathCheck iswells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. Supersedes 1. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. A: Microplate Preparation. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). Microplate Readers Biotek Synergy H1 Hybrid Microplate. SpectraMax Microplate Readers. Table of Contents. Microbiota DNA was. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. After being incubated in the dark at 60 ℃ overnight, the supernatants were centrifuged and optical densities were measured at 750, 665, 652 and 470 nm using the SpectraMax M5 microplate reader (Molecular Devices,. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Molecular Devices. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. 5–100mg/L (ValitaTiter) or 100–2000mg/L (ValitaTiter Plus). The spectrophotometric. Keywords. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. 6-384 (96- and 384-well with pipetting) 6-384. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Choose from a three- (M3), four-. For a higher . The SpectraMax M2 and M2e are versatile and reliable. . Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. M3-M5e_manual. 67. The chamber contains springs that position the cuvette in the proper alignment for a read. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit microplate reader. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). The cells were harvested on ice and. FlexStation™ III. No additional manuals are available. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. 6 for further experiment, so I'm confused which i would choose for OD value determination. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. When a feature or capability applies to only certain readers, this exception is noted. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Choose from a. The advantage of using labeled probes is detection specificity. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. 305 reference level saturation. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. The dual monochromator optics allow. D. Bookings are free, but you will need to use iLab to book. Self Service Available. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. E Models Only) 69. The cells were then treated to induce apoptosis. Examples: “Press carefully to engage,” “ Do not. 0 μL) instead of the sample solution was applied as control group, and. The. The PBS solution (10. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 1. Microplate Readers include the M3, M4, M5, and M5e models. This reader's dual monochromators for. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. The samples were boiled for 5 min with 1 ×. 5 12. TNF standard curve. 0 . PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe reader’s stated range. E Models. Go to Product Details. both cuvette-based and microplate reader applications The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. view more . Fifteen different automated tests are provided. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. com. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. book Page 7 Wednesday, July 7, 2010 2:09 PM. FlexStation™ II. Step 1. 5°C at 37°C. FlexStation™ II. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Plate Shaking. Plate chamber design of the SpectraMax category 1 microplate readers. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. The absorbance was recorded using a SpectraMax M5 microplate reader (Molecular Devices). 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. Lift up the lid over the chamber to insert or remove a cuvette. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Add to Cart. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Five preferred detection modes. ATP standard curves read on SpectraMax microplate readers. The bound bio-peptidomimetics were recognized by Eu 3+-SA (diluted using 1% BSA in TBS), and the fluorescence intensities were measured using a SpectraMax M5 microplate reader after adding 200 μL signal enhancement solution, which the parameters were set as the followed: excitation and emission wavelengths of 340 and 615 nm,. 00. Microplate Reader Documentation Version No. Add to Cart. Cavicide (Warehouse, Cat # 79300360). The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Anne T. Molecular Devices Spectramax M5 Microplate Reader. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Microplate Readers Biotek Epoch Microplate. Offer Ends In: 44 Days 10: 22: 56. The calculated LOQ values were 0. Spectramax Multi-Mode Microplate Reader Performance Specifications. free tracer decreases, resulting in. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. book Page 7 Wednesday, July 7, 2010 2:09 PM. Microplate formats. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Instrument settings are shown in Figure 3. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. anisotropy was measured on SpectraMax M5 microplate reader with 485 nm excitation and 535 nm emission after 60 min incubation at room temperature. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. Learn how to set up protocols, customize reports, export results, and more with this PDF document. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. Molecular Devices. The plate reader can measure multiple readouts, including UV and visible absorbance. 0 out of 5 $ 17,999 $ 16,995. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. 2 only) SpectraMax M-series Microplate readers. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Example: [Drawer]. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. SpectraMax® iD5. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. All reagents were brought to room temperature before use. SpectraMax® ABS Plus. . Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. When the user applies a set temperature of 37°C, the top is heated to 37. Use similar settings for other Molecular Devices readers with fluorescence detection mode. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. RAW 264. SpectraMax® M4. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry.